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The rings were mounted in an isolated organ chamber containing Krebs solution (pH 7.4) at 37 C and kept constant aerating with 95% O 2 /5% CO 2

Parenteral administration of minute amounts of endotoxins to humans elicits an inflammatory reaction typical of SIRS (fever or hypothermia, tachycardia, hyperpnea, leukocytosis, and hypotension), whereas larger amounts of administered endotoxins typically produce sepsis and shock [66, 67]

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